Dinitrophenol and valinomycin as uncouplers in isolated chloroplasts
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منابع مشابه
Influence of antimycin a and uncouplers on anaerobic photosynthesis in isolated chloroplasts.
Anaerobiosis depresses the light- and bicarbonate-saturated rates of O(2) evolution in intact spinach (Spinacia oleracea) chloroplasts by as much as 3-fold from those observed under aerobic conditions. These lower rates are accelerated 2-fold or more by the addition of 1 mum antimycin A or by low concentrations of the uncouplers 0.3 mm NH(4)Cl or 0.25 mum carbonyl cyanide m-chlorophenylhydrazon...
متن کاملIsolated Chloroplasts
1. Chloroplasts prepared by the non-aqueous technique will, after fragmentation by ultrasonic treatment, incorporate [2-14C]mevalonic acid into phytoene, the first C40 compound formed in the biosynthetic sequence to coloured carotenoids. 2. With suspensions containing 3*5mg. of chlorophyll, the optimum amounts of cofactor required were ATP (10,umoles), magnesium chloride (20,umoles) and glutath...
متن کاملPhotosynthesis and phosphorylation of light-harvesting chlorophyll a/b-protein in intact chloroplasts Effects of uncouplers
Protein phosphorylation in isolated, intact pea chloroplasts was measured during the onset of COZ-dependent 02 evolution. Total incorporation of 32P (from 32Pi) into light-harvesting chlorophyll a/b-protein was found to be less sensitive than 02 evolution to inhibition by the uncouplers FCCP and NH&l. It is concluded that changes in the rate of ATP synthesis cannot affect protein phosphorylatio...
متن کاملPhotophosphorylation Associated with Photosystem II: III. Characterization of Uncoupling, Energy Transfer Inhibition, and Proton Uptake Reactions Associated with Photosystem II Cyclic Photophosphorylation.
A number of uncouplers and energy transfer inhibitors suppress photosystem II cyclic photophosphorylation catalyzed by either a proton/electron or electron donor. Valinomycin and 2,4-dinitrophenol also inhibit photosystem II cyclic photophosphorylation, but these compounds appear to act as electron transport inhibitors rather than as uncouplers. Only when valinomycin, KCl, and 2,4-dinitrophenol...
متن کاملSucrose as a product of photosynthesis in isolated spinach chloroplasts.
Sucrose has been detected as a seasonal photosynthetic product in spinach chloroplast preparations. Sucrose when present accounted for up to 30% of the CO(2) fixed. Experiments in which sucrose was formed have been compared with experiments in which it was not formed, and a possible control mechanism for sucrose synthesis is discussed.
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1968
ISSN: 0014-5793
DOI: 10.1016/0014-5793(68)80008-0